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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
10/08/2018 |
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Data da última atualização: |
24/09/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NICIURA, S. C. M.; CRUVINEL, G. G.; MORAES, C. V.; DONATONI, F. A. B.; MALAGO JUNIOR, W.; BENAVIDES, M. V.; CHAGAS, A. C. de S. |
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Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; Giovanna Gabrielle Cruvinel, UNICEP; Caroline Valério Moraes, UFSCar; FLAVIA ALINE BRESSANI DONATONI, CPPSE; WILSON MALAGO JUNIOR, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
PCR-based genotyping of SNP markers in sheep. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Biology Reports, v.45, n.4, p.651-654, aug. 2018. |
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ISSN: |
1573-4978 |
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DOI: |
https://doi.org/10.1007/s11033-018-4206-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Single nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays. |
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Palavras-Chave: |
PCR RFLP; Resistência nematódeo gastrintestinal; Tetra primer ARMS PCR. |
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Thesagro: |
Marcador Molecular; Ovino. |
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Thesaurus Nal: |
Genome. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02300naa a2200289 a 4500
001 2094070
005 2018-09-24
008 2018 bl uuuu u00u1 u #d
022 $a1573-4978
024 7 $ahttps://doi.org/10.1007/s11033-018-4206-8$2DOI
100 1 $aNICIURA, S. C. M.
245 $aPCR-based genotyping of SNP markers in sheep.$h[electronic resource]
260 $c2018
520 $aSingle nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays.
650 $aGenome
650 $aMarcador Molecular
650 $aOvino
653 $aPCR RFLP
653 $aResistência nematódeo gastrintestinal
653 $aTetra primer ARMS PCR
700 1 $aCRUVINEL, G. G.
700 1 $aMORAES, C. V.
700 1 $aDONATONI, F. A. B.
700 1 $aMALAGO JUNIOR, W.
700 1 $aBENAVIDES, M. V.
700 1 $aCHAGAS, A. C. de S.
773 $tMolecular Biology Reports$gv.45, n.4, p.651-654, aug. 2018.
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Embrapa Pecuária Sudeste (CPPSE) |
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| 63. |  | OLIVEIRA, I. P. de; BALBINO, L. C.; KLUTHCOUSKI, J.; YOKOYAMA, L. P.; MAGNABOSCO, C. U. Avaliação preliminar da correção e adubação utilizadas no processo de reforma de pastagem pelo Sistema Barreirão com consórcio arroz-brachiaria. In: REUNIÃO NACIONAL DE PESQUISA DE ARROZ, 6., 1998, Goiânia. Perspectivas para a cultura dos arroz nos ecossistemas de várzeas e terras altas: resumos expandidos. Goiânia: Embrapa-CNPAF, 1998. v. 1. p. 132-135. Embrapa-CNPAF. Documentos, 85).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 70. | | OLIVEIRA, O. de; OLIVEIRA, I. P. de; URQUIAGA, S.; ALVES, B. J. R.; BODDEY, R. M. Chemical and biological indicators of decline/degradation of Brachiaria pastures in the Brazilian Cerrado. In: INTERNATIONAL SYMPOSIUM SOIL FUNCTIONING UNDER PASTURES IN INTERTROPICAL AREAS, 2000, Brasilia, DF. Extended abstracts. Planaltina, DF: Embrapa Cerrados, 2000. 5 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 71. | | STONE, L. F.; MOREIRA, J. A. A.; PINHEIRO, B. da S.; OLIVEIRA, I. P. de. Consumo de água da consorciação arroz de terras altas Brachiaria brizantha em comparação com o arroz solteiro. In: CONGRESSO DA CADEIA PRODUTIVA DE ARROZ, 1.; REUNIÃO NACIONAL DE PESQUISA DE ARROZ - RENAPA, 7., 2002, Florianópolis. Anais... Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2002. p. 328-330. (Embrapa Arroz e Feijão. Documentos, 134).| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 74. | | OLIVEIRA, I. P. de; BALBINO, L. C.; KLUTHCOUSKI, J.; YOKOYAMA, L. P.; MAGNABOSCO, C. U. Influência do gesso e do calcário no consórcio arroz - Brachiaria brizantha. In: REUNIÃO NACIONAL DE PESQUISA DE ARROZ, 6., 1998, Goiânia. Perspectivas para a cultura dos arroz nos ecossistemas de várzeas e terras altas: resumos expandidos. Goiânia: Embrapa-CNPAF, 1998. V. 1. P. 128-131. (Embrapa-CNPAF. Documentos, 85).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 243 | |
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